plasmid dna and fugene hd transfection reagent  (Promega)

Journal: Annals of Medicine and Surgery

Article Title: Brain tumors and induced pluripotent stem cell technology: a systematic review of the literature

doi: 10.1097/MS9.0000000000002760

Figure Lengend Snippet: Basic characteristics of final selected studies

Article Snippet: 13 , Haag et al ., 2021 , USA , Animal study/mice/in vivo , DIPG , Transfection non-viral/hiPSC , Mice xenograft , Plasmid-FuGene HD transfection reagent (Promega) , Modeling/Astroglial and oligodendroglial differentiation , This study showed increase in neural stem cell proliferation, and a viability reduction in H3.3-K27M DIPG cells reported in overexpression of K27M specifically in H3.3.

Techniques: Transduction, Transfection, In Vivo, Virus, In Vitro, Activity Assay, Plasmid Preparation, Transformation Assay, Derivative Assay, Mutagenesis, Activation Assay, Over Expression, Introduce, Expressing, Generated, Comparison

Journal: Annals of Medicine and Surgery

Article Title: Brain tumors and induced pluripotent stem cell technology: a systematic review of the literature

doi: 10.1097/MS9.0000000000002760

Figure Lengend Snippet: Method of generating iPSCs surveyed in all 23 selected studies. Among 23 selected studies, 14 studies administered transfection methods and seven studies used transduction methods. The details on the number of studies in each category are illustrated. Illustrations used elements from BioRender.com (2021) ( https://app.biorender.com/biorender-templates/ , accessed on November 2023). iPSC, induced pluripotent stem cell.

Article Snippet: 13 , Haag et al ., 2021 , USA , Animal study/mice/in vivo , DIPG , Transfection non-viral/hiPSC , Mice xenograft , Plasmid-FuGene HD transfection reagent (Promega) , Modeling/Astroglial and oligodendroglial differentiation , This study showed increase in neural stem cell proliferation, and a viability reduction in H3.3-K27M DIPG cells reported in overexpression of K27M specifically in H3.3.

Techniques: Transfection, Transduction

Journal: Scientific Reports

Article Title: The study on circRNA profiling uncovers the regulatory function of the hsa_circ_0059665/miR-602 pathway in breast cancer

doi: 10.1038/s41598-024-71505-0

Figure Lengend Snippet: Hsa_circ_0059665 is associated with the prognosis of breast cancer and inhibits the proliferation, migration, and invasion of breast cancer cells. ( A ) FISH analysis was used to observe the level of hsa_circ_0059665 in breast cancer tissue microarrays. ( B ) Survival analysis indicates that decreased expression of hsa_circ_0059665 is associated with poor prognosis in breast cancer patients. ( C ) Expression levels of hsa_circ_0059665 in breast cancer cells. * p < 0.05, ** p < 0.01. ( D ) Level of hsa_circ_0059665 in breast cancer cells after transfection with hsa_circ_0059665 overexpression plasmid was observed using qRT-PCR. *** p < 0.001. ( E , F ) CCK8 assay showed that overexpression of hsa_circ_0059665 significantly inhibited proliferation of MDA-MB-231 and MDA-MB-453 cells, respectively. *** p < 0.01. ( G , H ) The Transwell assay revealed that upregulation of hsa_circ_0059665 inhibited the migratory and invasive abilities of MDA-MB-231 and MDA-MB-453 cells, respectively. * p < 0.05, ** p < 0.01.

Article Snippet: For circRNA transfection, a plasmid of 3 μg was transfected using FuGENE HD transfection reagent (Promega, USA).

Techniques: Migration, Expressing, Transfection, Over Expression, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, Transwell Assay